Data on Cytoprotective and Anti-inflammatory activities of seagrass Halophila beccarii

Fig. 1. Inhibition of protein denaturation activity by seagrass methanol extract & Diclofenac. Fig.4. NO production in LPS stimulated Raw macrophages. The measurement of nitrite concentration in the culture supernatant of the seagrass extract pretreated RAW 264.7 macrophages. In the measurement of nitrite, the cell line with only LPS showed a more significant increase of 100µM/mL than the normal group. The production of nitrite was significantly reduced with increasing the concentration of seagrass methanol extract. Fig.5. (A) The effect of seagrass extract on the expression of COX-1 and COX-2 in LPS stimulated raw 264.7 macrophage cells assessed by Western blotting. After pre-treatment with the indicated concentrations of methanol extract, cells were stimulated with LPS (1μg/ml) for 4h. Equal loading was confirmed by stripping the blot and reporting it for GAPDH. Data shown are representative expression patterns from triplicate independents. (B) The densitometric data of COX-2 protein expression representing the relative density of the Western blot bands normalized to GAPDH