Data on Cytoprotective and Anti-inflammatory activities of seagrass Halophila beccarii

Fig. 1. Inhibition of protein denaturation activity by seagrass methanol extract & Diclofenac. Fig.4. NO production in LPS stimulated Raw macrophages. The measurement of nitrite concentration in the culture supernatant of the seagrass extract pretreated RAW 264.7 macrophages. In the measurement of nitrite, the cell line with only LPS showed a more significant increase of 100µM/mL than the normal group. The production of nitrite was significantly reduced with increasing the concentration of seagrass methanol extract. Fig.5. (A) The effect of seagrass extract on the expression of COX-1 and COX-2 in LPS stimulated raw 264.7 macrophage cells assessed by Western blotting. After pre-treatment with the indicated concentrations of methanol extract, cells were stimulated with LPS (1μg/ml) for 4h. Equal loading was confirmed by stripping the blot and reporting it for GAPDH. Data shown are representative expression patterns from triplicate independents. (B) The densitometric data of COX-2 protein expression representing the relative density of the Western blot bands normalized to GAPDH图1 海草甲醇提取物与双氯芬酸（Diclofenac）对蛋白质变性活性的抑制作用。图4 脂多糖（LPS）刺激RAW 264.7巨噬细胞后的一氧化氮生成情况：检测经海草提取物预处理的RAW 264.7巨噬细胞培养上清液中的亚硝酸盐浓度。仅用LPS处理的细胞组较正常组的亚硝酸盐浓度显著升高至100μM/mL；随着海草甲醇提取物浓度升高，亚硝酸盐生成量显著降低。图5 (A) 采用蛋白质印迹法（Western blotting）检测海草提取物对LPS刺激的RAW 264.7巨噬细胞中环氧合酶-1（COX-1）与环氧合酶-2（COX-2）表达的影响。将细胞以指定浓度的甲醇提取物预处理后，用1μg/mL的LPS刺激4小时。通过对印迹膜进行剥离并重检测甘油醛-3-磷酸脱氢酶（GAPDH）的表达，以确认各组上样量均一。所示数据为三次独立重复实验的代表性表达图谱。(B) COX-2蛋白表达的光密度定量数据，代表以GAPDH为参照标准化后的免疫印迹条带相对灰度值。