Gene expression profile of germinal center B cells lacking Miz1 interaction with Myc

Splenic germinal center B cells (B220pos, CD19pos, CD38 low, FAS high) were isolated from animals 10 days sheep red blood cell immunised animals using flow cytometry cell sorter. Animals carry a combination of alleles that allows germinal center B cell deletion of endogenous Myc allele and simultaneous conditional overexpression of human MYC in its wild-type for or mutant form in which a valine in residue 394 has been substituted by an aspartic acid. The mouse WG-6 v2.0 expression beadchip array (Illumina) was used to determine gene expression of germinal center B cells