Model parameters used in simulations.
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The rate constant is set equal to k3 throughout the work, whereas , and may differ from their native counterparts.1Calculated from λcat [T], using λcat = 1.7×109 M−1 min−1[34] and [T] = 0.16 nM (equivalent to 250 toxin molecules in the cytosol of a cell, with a cell cytosol volume equal to 2.6 pL [35].2Obtained by the ratio of [EF2]t : [R]t = 1.2∶ 1 [37] using the estimated [EF2]t.3Calculated as k1 = kakGTP/(kd+kGTP) = 150*250/(140+250) = 96 s−1.4Taken as the rate constant for relocking [8], [9].5Based on [GTP]:[GDP] = 10∶1 [32].
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2015-12-02
