Selective advantage of epigenetically disrupted cancer cells via phenotypic inertia [ChIP-Seq]

Mutations in over one hundred genes, belonging to diverse classes of epigenetic regulators, resulted in significant fitness advantage under nutrient starvation and acidic conditions, suggesting that disruption of epigenetic control, at multiple levels of the regulatory network, promotes broad resistance of cells to stressful environments. To uncover the molecular mechanisms of such phenomenon, and to understand how loss of epigenetic regulators gives advantage during stress, we plan to examine how the chromatin landscape changes in nutrient-deprived conditions. Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27me3, H3K27Ac, H3K4me3 and H3K4me1 in PDX MEXF 2090 cells.