Stable epigenetic programming of effector and central memory CD4 T cells occurs within 7 days of antigen exposure in vivo (ATAC-seq)

T cell immunological memory is established within days of an infection but little is known about the in vivo changes in gene regulatory networks which account for the ability of memory T cells to respond more efficiently to secondary infections. To decipher the timing and nature of immunological memory we performed genome-wide analyses of epigenetic and transcriptional changes in a mouse model generating antigen-specific T cells. Overall design: ATAC-seq of naïve CD4 T cells, in vivo generated antigen specific Th1 cells 7, 28 and 56 dpi and and antigen specific Tcm and Tem cells 7 dpi