Metabolomic Profiling of CD4+ T cells into Tfh Cells in Transplant Arteriosclerosis

This dataset comprises metabolomic profiling of CD34 lineage cell differentiation into Tfh cells in transplant arteriosclerosis. Metabolic signatures were analyzed using ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). Both untargeted and targeted metabolomics approaches were employed to assess metabolic flux in CD4+ T cells at different differentiation stages, with specific focus on mitochondrial one-carbon metabolism. CD4+ T cells were isolated from allograft arteries and lymphoid tissues , and metabolite extraction was performed using 80% methanol, followed by centrifugation and filtration. The LC-MS analysis was conducted in both positive and negative ionization modes, and metabolite identification was performed based on MS1 and MS2 spectra using in-house and public databases. Data processing included peak alignment and normalization using internal standards.本数据集涵盖移植性动脉硬化中CD34谱系细胞向滤泡辅助性T细胞（T follicular helper cells，Tfh）分化的代谢组学分析数据。研究采用超高效液相色谱-高分辨质谱联用技术（UHPLC-HRMS）对代谢特征展开分析。本研究同时采用非靶向与靶向代谢组学方法，对不同分化阶段CD4+ T细胞的代谢流进行评估，重点关注线粒体一碳代谢。实验所需CD4+ T细胞分离自同种异体移植动脉与淋巴组织，代谢物提取采用80%甲醇溶液，随后经离心与过滤处理。液相色谱-质谱分析采用正、负两种电离模式开展，基于一级质谱（MS1）与二级质谱（MS2）谱图，并结合内部及公共数据库完成代谢物鉴定。数据处理包括利用内标进行峰对齐与归一化操作。