Infection and host-feeding patterns of West Nile virus vectors varies by urban greenspace composition

Greenspaces are integral to the urban environment, enhancing climate resilience and providing several additional environmental, social, and public health benefits, yet the extent to which they affect vector-borne disease transmission is not clear. We explored this question in the context of mosquito and West Nile virus (WNV) host populations, infection rates, and foraging behavior. We collected mosquitoes from greenspaces along a transect spanning a range of urban development. Mosquitoes were tested for West Nile virus, and the host species that were fed on was determined for blood-fed specimens. Bird counts and camera traps were used to assess avian and mammal species availability. We explored landcover associations with vector abundance, vector infection prevalence, host-feeding pattern,s and host community composition. Different components that contribute to WNV transmission intensity responded to different landcover variables. Abundance of Culex mosquitoes was most strongly tied to ..., , # Data from: Infection and host-feeding patterns of West Nile virus vectors varies by urban greenspace composition Dataset DOI: [10.5061/dryad.nk98sf84h](10.5061/dryad.nk98sf84h) ## Description of the data and file structure These files contain data required to reproduce analyses in the associated paper examining how the composition and extent of urban greenspace influences the abundance, host feeding behavior and West Nile virus (WNV) infection prevalence of *Culex (Cx.)* spp. mosquitoes at ten locations along a gradient of urban development intensity in the Greater Chicago area. Mosquitoes were collected weekly from multiple traps at each location, 25 June to 25 September 2019. Unfed, female specimens were pooled and tested by RT-PCR for the presence of WNV RNA. Vertebrate DNA was extracted from blood engorged *Culex (Cx.)* spp. females and sequenced to determine host identity. Point count surveys and camera traps were used at each site to estimate relative abundance of avian and m...,城市绿地是城市环境不可或缺的组成部分，既能提升气候韧性，还可带来多项额外的环境、社会与公共健康效益，但其对虫媒疾病传播的影响程度仍不明确。本研究以蚊虫、西尼罗病毒（West Nile Virus, WNV）宿主种群、感染率及觅食行为为研究对象，探讨了这一科学问题。研究团队沿覆盖不同城市开发程度的样带，在城市绿地中采集蚊虫样本；对所有采集的蚊虫开展西尼罗病毒检测，并针对吸血标本确定其吸食的宿主物种；同时通过鸟类计数与相机陷阱法评估鸟类与哺乳动物的物种可获得性。本研究还探讨了土地覆盖与媒介丰度、媒介感染率、宿主取食模式以及宿主群落组成之间的关联。 影响西尼罗病毒传播强度的不同组分，对不同土地覆盖变量的响应存在差异。库蚊属（*Culex* (*Cx.*)）蚊虫的丰度与……关联最为紧密。 # 数据来源：《城市绿地组成差异影响西尼罗病毒媒介的感染与宿主取食模式》 数据集DOI：10.5061/dryad.nk98sf84h ## 数据与文件结构说明 本数据集包含复现相关论文分析所需的全部数据，该论文探讨了大芝加哥地区10个沿城市开发强度梯度布设的样点中，城市绿地的组成与覆盖范围如何影响库蚊属（*Culex* (*Cx.*)）蚊虫的丰度、宿主取食行为以及西尼罗病毒（WNV）感染率。 研究团队于2019年6月25日至9月25日期间，每周在每个样点的多个诱捕器中采集蚊虫样本。未吸血的雌性标本经混合后，通过逆转录聚合酶链式反应（Reverse Transcription Polymerase Chain Reaction, RT-PCR）检测西尼罗病毒RNA的存在。从吸血的库蚊属雌性标本中提取脊椎动物DNA并进行测序，以确定其宿主种类。各调查点均采用样点计数法与相机陷阱法评估鸟类与哺乳动物的相对丰度……