Anticoagulants and their effects on the hematological and biochemical parameters of yellow-spotted amazon river turtle

ABSTRACT: Knowledge of suitable methods and reagents for assessing the health condition of specimens of a given species is essential. The present study evaluated the efficacy of the heparin anticoagulants 5,000 I.U. mL-1, Na2EDTA, and K3EDTA on the blood parameters of yellow-spotted amazon river turtle Podocnemis unifilis, employing different solutions for red blood cells count. The use of the various anticoagulants evaluated after 10 hours of storage efficiently inhibited coagulation in blood samples from P. unifilis. An increased number of erythrocytes was observed with the use of K3EDTA 5% when compared with heparin. Statistically significant changes in the erythrocyte number were observed with the use of the different solutions. Solutions which featured sodium citrate and formaldehyde in their composition, allowed erythrocytes counting up to 120 hours after blood collection, without a change in values. The use of the heparin anticoagulants 5,000 I.U. mL-1, Na2EDTA 3%, Na2EDTA 5%, K3EDTA 3% was recommended in the hematological analysis of P. unifilis. Also recommended was the use of the formaldehyde-citrate solution containing 1.9 g of sodium citrate and 1.0 mL of formaldehyde (in 50 mL of distilled water) to perform red blood cells counts in yellow-spotted amazon river turtle.摘要：掌握适用于评估特定物种标本健康状况的方法与试剂，是开展相关实验研究的必要基础。 本研究以黄点亚马逊河龟（Podocnemis unifilis）为实验对象，针对5000 I.U./mL肝素抗凝剂（heparin anticoagulants）、乙二胺四乙酸二钠（Na₂EDTA）与乙二胺四乙酸三钾（K₃EDTA）的抗凝效果，结合多种红细胞计数溶液对其血液参数的影响开展了评估。 经10小时储存后，本研究所评估的各类抗凝剂均可有效抑制黄点亚马逊河龟血液样本的凝固。与肝素组相比，使用5% K₃EDTA时可观测到红细胞计数显著升高；不同红细胞计数溶液的使用会导致红细胞计数出现具有统计学意义的显著变化。以柠檬酸钠与甲醛为主要成分的计数溶液，可在采血后120小时内完成红细胞计数且检测结果无明显波动。 针对黄点亚马逊河龟的血液学分析，推荐使用5000 I.U./mL肝素抗凝剂、3% Na₂EDTA、5% Na₂EDTA与3% K₃EDTA；此外，推荐采用含1.9g柠檬酸钠与1.0mL甲醛（溶于50mL蒸馏水）的甲醛-柠檬酸钠溶液，开展该物种的红细胞计数检测。