On/Off targets INDEL analysis by Next Generation Sequencing
收藏NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP328343
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资源简介：
SpCas9 INDEL frequency was evaluated at both the on- and off-target sites of guideRNA targeting either the murine Rhodopsin or Albumin by Next Generation Sequencing. Minimal INDELs were found at the off target sites evaluted, while SpCas9 activity was predominantly on-target. Overall design: Three gRNA- and 2 scramble control-treated samples of either retina or liver were used to amplify the gRNA on-target and the top 10 off-target sites.
创建时间：
2021-11-12
