Transcriptome data of zebrafish with glula knockdown and glulb knockdown

To investigate global changes in gene expression following the knockdown of glula or glulb in zebrafish, we employed RNA-sequencing to analyze the whole-genome transcriptomes of glula or glulb knockdown zebrafish. Three-day-old wild-type zebrafish and zebrafish injected with glula-MO and glulb-MO were prepared for analysis, with three independent replicates performed for each treatment. The total RNA extraction was performed using TRIzol reagent (Invitrogen, United States) according to the manufacturer's protocol. Subsequently, the quality and quantity of total RNAs were assessed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, United States), ensuring that the RIN values exceeded 7.0. The RNA samples were subsequently submitted to GENEWIZ Science (Suzhou, China) for deep sequencing using an Illumina Hiseq2500 platform.