Precision cutaneous stimulation in freely moving mice

Somatosensation connects animals to their immediate environment, shaping critical behaviors essential for adaptation, learning, and survival. Probing the relationships between somatosensory inputs and behavior in mice presents substantial challenges, primarily due to the practical difficulties of delivering stimuli to the skin during movement. To address this problem, we have developed a system for precise cutaneous stimulation of mice as they walk and run through environments. The system employs real-time body part tracking and targeted optical stimuli, offering precision while preserving the naturalistic context of the behaviors studied, thereby overcoming the traditional trade-offs between precision and animal behavior. We demonstrate the system from nociceptive testing conducted in standard small chambers to behavior in large complex environments, such as mazes. We observed that cutaneous inputs evoke rapid responses, which modify behavior when stimuli are applied during motion. Thi..., , , # Data from: Precision cutaneous stimulation in freely moving mice

[https://doi.org/10.5061/dryad.rr4xgxdhs](https://doi.org/10.5061/dryad.rr4xgxdhs)

Corresponding Author Contact Information

```
Name: Liam E. Browne
Institution: University College London (UCL)
Email: [e-mail]
```

First Author Contact Information

```
Name: Isobel Parkes
Institution: University College London (UCL)
Email: [e-mail]
```

#### Dataset Overview:

This dataset contains files used for generating key figures, which demonstrate the development of closed-loop somatosensory stimulation in freely moving mice. Each `.csv` or `.pkl The` file corresponds to specific panels and figures in the manuscript, providing data for further analysis and validation.

#### Dataset File List:

1\. Fig2_DLC_TailBase.csv
2\. Fig2_DLC_LeftHindPaw.csv
3\. Fig2K_HitAccuracy.csv
4\. Fig2L_LaserMAE.csv
5\. Fig2I_DLC_84R.csv
6\. Fig5F_ActionRewardTimestamps.pkl
7\. Fig5H_CorridorSpeeds.csv
8\. Fig5H_AngleStd.csv
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