Characterization of postsynaptic glutamate transporter functionality in the zebrafish retinal first synapse across different wavelengths

In the zebrafish retina, incident light undergoes wavelength-dependent processing encompassing mechanisms such as color opponency, contrast enhancement, and motion detection prior to neural transmission to the brain proper. In darkness, photoreceptors continuously release glutamate into the synaptic cleft, a process that diminishes in response to increased light intensity, thereby conveying visual signals to ON and OFF bipolar cells. Specifically, in zebrafish, the ON pathway signal transduction is mediated by metabotropic glutamate receptor 6b (mGluR6b) and Excitatory Amino Acid Transporters (EAATs). Here we demonstrate that knockout of eaat5b and eaat7 disrupts electroretinogram responses to short and long-wavelength stimuli while preserving middle-wavelength responses, suggesting wavelength-specific roles. We found differential expression of EAAT5b and EAAT7 in the outer plexiform layer, particularly in the strike zone, crucial for prey capture, supporting task specific involvement o..., , , # Project Title: Characterization of postsynaptic glutamate transporter functionality in the zebrafish retinal first synapse across different wavelengths

Date of data collection: 2019-2024

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General Information

This dataset contains all source data related to the manuscript \"Characterization of postsynaptic glutamate transporter functionality in the zebrafish retinal first synapse across different wavelengths.\"

The data includes:

* Electroretinogram (ERG) recordings for single and double mutant zebrafish larvae.
* Confocal microscopy images and manual intensity analysis for full retinal mapping.
* Scripts, 3D models (STL files), and spectrometer readings for a custom UV hunting behavioral setup and OMR behavioral assays.
* Raw behavioral tracking data for UV hunting assays (wild type and mutants).
* Raw behavioral tracking data for optomotor response (OMR) assays.

The dataset is organized into folders corresponding to the figures in the main publication.
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