Significant correlations between different indicators of the production of ROS and membrane intactness and mitochondrial functionality.

** p<0.01, n.s non-significant Mitosox+ spermatozoa show mitochondrial production of superoxide radical, H2DCFDA—percentage of spermatozoa showing hydrogen peroxide production, CellRox (Live Cells)—percentage of live spermatozoa showing ROS production, HE—percentage of spermatozoa showing superoxide production, Intact sperm—percentage of spermatozoa with completely intact membranes, YoPro+—percentage of spermatozoa with intact membranes but with increased permeability, JC- high percentage of spermatozoa with high mitochondrial membrane potential. Significant correlations between different indicators of the production of ROS and membrane intactness and mitochondrial functionality.