Intracellular infection of ecologically important diatom species by an evolutionary distinct relative of the Fungi

Data and R script from the publication "Intracellular infection of ecologically important diatom species by an evolutionary distinct relative of the Fungi":

-plotting_and_testing_association.r: R script for the s.tatistical testing of NCLC1-diatom associations.

-OSD_protist_ASV_table.csv: Amplicon Sequence Variant (ASV) table, comma separated. Lists the distribution of 7766 ASVs across 145 samples collected during the Ocean Sampling Day (OSD) 2014.

-OSD_protist_ASV_sequences.fasta: fasta nucleotide file of the 7766 V4-18S ASVs sequenced as part of OSD and classified as unicellular eukaryotes ('protists'; re-processed in the present publication via DADA2).

-sparCC_analysis.tar.gz: gzip'd archive with results from co-occurrence analyses ran on the protist OSD V4-18S dataset using sparCC (OSD_protist_ASV_sparCC_cor.csv: correlation output; OSD_protist_ASV_sparCC_100perm_pvals_twosided.csv: results of two-sided t-tests based on 100 sparCC permutations).

-holomycota_alignment.fasta: multiple sequence alignment of reference holomycota 18S.

-holomycota_MLtree_100nonparamboot.nwk: 18S maximum likelihood tree inferred using IQ-Tree; branch supports assessed with 100 non-parametric bootstrap replicates.