BRET2 saturation assay fitting results.

BRET2 data from saturation assays were fitted using the following equation for dimers: BRET = BRETmax(1/(1+AD50/X)) where X is the ratio of acceptor (A; Receptor-GFP2) to donor (D; Receptor-RLuc8) molecules. The BRETmax is the maximal BRET obtained for a given pair and AD50 value corresponds to the A/D ratio providing 50% of the BRETmax. The best-fit parameters and standard errors were derived from the data presented in Fig. 3. Fitting parameters were compared using Welch’s t-test. Statistical analysis shows that AD50 and BRETmax values differ significantly (p≤0.05) between all of the tests.BRET2 saturation assay fitting results.