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Handling of spurious sequences dictates the outcome of high-throughput 16S rRNA gene amplicon profiling

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NIAID Data Ecosystem2026-03-11 收录
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Handling of artefact sequences during bioinformatic processing of 16S rRNA gene amplicon data requires careful attention. A threshold of relative abundance of 0.25% seems more appropriate than the commonly used singleton removal approach, although study-specific analysis strategies are mandatory. We propose the concept of normalized richness, the use of which will help comparison of results across studies.

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2019-12-20
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