Xinong Saanen dairy goat Transcriptome with FSH treatment. Capra hircus

Genes differentially expressed in ovaries of goats treated with once or repeated estrus synchronization (ES) were determined in a total of 24 female Xinong Saanen dairy goats. The goats were selected randomly divided into two groups, one group was received thrice ES treatments at fortnight intervals (repeated group), the other was only treated once on the same day as the third ES for repeated group (control group) during the breeding season. Ovaries of three estrus goats from either group were collected for morphological examination and transcriptome sequencing, while the rest of goats were artificially inseminated twice with about 100 million normal motile sperm. In this study, RNA-Seq technology was employed to analyze the DEGs in goat ovaries treated with once gonadotropins or triplet gonadotropins in a genome-wide level. We found that several genes were differentially expressed between the two libraries.