Identifying differentially expressed genes under heat stress and developing molecular markers in orchardgrass (Dactylis glomerata L.) through transcriptome analysis

Orchardgrass (Dactylis glomerata L.) is a long-lived, cool-season forage grass that is commonly used for hay production. Despite its economic importance, orchardgrass genome remains relatively unexplored. In this study, we used Illumina RNA sequencing to identify gene-associated molecular markers, including simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), as well as heat stress-induced differentially expressed genes (DEGs) in two orchardgrass genotypes, ‘Baoxing’ (heat resistant) and ‘01998’ (heat susceptible). Approximately 163 million high-quality trimmed reads were generated from 207 million raw reads using the Illumina HiSeq 2000 platform. A total of 126 846 unigenes were obtained after de novo assembly of the trimmed reads, and 40 078 unigenes were identified as coding sequences (CDSs). Based on the assembled unigenes, 669 300 high-quality SNPs, including 416 099 transitions and 257 736 transversions, were contained in 75 875 unigenes. In addition, a total...鸭茅草（Dactylis glomerata L.）是一种长寿命冷季型饲草，常用于干草生产。尽管其经济价值显著，但鸭茅草的基因组研究仍相对匮乏。本研究采用Illumina RNA测序技术，在两个鸭茅草基因型——‘宝兴’（耐热型）和‘01998’（热敏感型）中，鉴定与基因相关的分子标记，包括简单序列重复（Simple Sequence Repeats, SSRs）和单核苷酸多态性（Single Nucleotide Polymorphisms, SNPs），以及热胁迫诱导的差异表达基因（Differentially Expressed Genes, DEGs）。本研究通过Illumina HiSeq 2000平台，从2.07亿条原始reads中生成约1.63亿条高质量过滤reads。经从头组装后，共获得126846个单基因簇（unigene），其中40078个被鉴定为编码序列（Coding Sequences, CDSs）。基于组装得到的单基因簇（unigene），在75875个单基因簇（unigene）中共包含669300个高质量SNPs，其中包括416099个转换型变异和257736个颠换型变异。此外，总计……