JASMONATE-ZIM DOMAIN proteins engage Polycomb chromatin modifiers to modulate Jasmonate signaling in Arabidopsis [RNA-Seq]

JASMONATE-ZIM DOMAIN (JAZ) proteins are key regulators in the JA signaling pathway and function to repress the expression of JA-responsive genes. We found that JAZ proteins directly interact with several chromatin-associated Polycomb proteins to mediate repressive chromatin modifications at part of JA-responsive genes and thus their transcriptional repression in Arabidopsis. For RNA-seq analysis, total RNAs were extracted from 6 day-old seedlings treated with or without 5-µm JA for 3hr. Three samples including WT (mock), lhp1 (mock), and WT (JA) were analyzed for RNA-seq (each sample with three biological replicates).