Expression data from the visceral adipose tissues of Lgr4 mutant (KO) and wild-type (WT) mice

Lgr4 acts as a key factor to regulate energy expenditure and body weight. Visceral fat (epidydimal white adipose tissue, eWAT) show a significant reduction in Lgr4 mutant mice than littermate controls, especially in high-fat diet (HFD) condition. A white-to-brown fat switch of eWAT in Lgr4 mice was reported by our group. However, the whole-genome expression profile of eWAT has not been comphrehensively studied. We used microarrays to reveal the potential novel factors underlying browning process. eWAT tissues from both Lgr4 mutant and WT mice under chow diet and HFD diet were sampled for RNA extraction and hybridization on Affymetrix microarrays.