Sample preparation strategies for HMGB1

Investigating the outcomes of antibody-free sample preparation strategies for LC-MS/MS analysis of high mobility group 1 (HMGB1) protein in human plasma. The number of peptides and other proteins identified was found by searching the Global Proteome Machine (GPM) database using these Mascot generic format-files.本研究旨在探究无抗体样品前处理策略应用于人血浆中高迁移率族蛋白1（HMGB1）的液相色谱-串联质谱（LC-MS/MS）分析的实验效果。通过使用Mascot通用格式文件检索全球蛋白质组机器（GPM）数据库，对所鉴定得到的肽段及其他蛋白质的数量进行统计。