RNA BS-seq of Nocodazole treated HeLa cells with NSUN family member deficiency

m5C accumulates on mRNAs in Nocodazole (Noc) treated cells. To figure out which m5C writer is responsible for these m5C sites, we generated three KO cell lines (NSUN2, NSUN5, and NSUN6) with CRISPR induced mutagenesis. We also used siRNA to suppress the expression of Nop2 (NSUN1) in wild-type HeLa cells. Then we performed mRNA BS-seq on the cells harvested after 48 hours Noc-treatment (for siRNA experiments, cells were treated with siRNA for 48 hours first). Overall design: RNA BS-seq of mRNA from human cell lines (NSUN2 KO, NSUN5 KO, NSUN6 KO, Nop1 KD). For each type of cells, one biological replicate was performed.