Fluorescent measurements of the endotoxin adsorption efficiency

We present the technique for the measurement the LPS statical adsorption on the particles in the area of relatively low concentrations. Direct photon count allows us to detect LPS concentration securely from 100 pM. Using this technique we demonstrate how preequilibration pH affects the results. Raw adsorption data sufficient for building adsorption isotherm is also presented for two sorbents.我们提出了一种适用于相对低浓度区间的颗粒表面脂多糖（LPS）静态吸附检测技术。采用直接光子计数法，我们可稳定可靠地从100皮摩尔（pM）浓度起检出LPS。借助该技术，我们验证了预平衡pH值对实验结果的影响规律。此外，我们还提供了两种吸附剂的原始吸附数据，这些数据足以用于构建吸附等温线。