RNAseq data: Reporter-Based Quantification of NF-κB and ISGF3 Signaling in BLaER1 Macrophages

This repository contains raw and processed RNA sequencing (RNA-seq) data generated to characterize transcriptional changes during BLaER1 B cell-to-macrophage trans-differentiation and inflammatory stimulation. Human B cell Leukemia C/EBPα Estrogen Receptor clone 1 (BLaER1) eGFP-/- cells were cultured in RPMI 1640 (Thermo Fisher) supplemented with 100 U/mL penicillin (Gibco), 100 µg/mL streptomycin (Gibco), 2 mM L-glutamine (Gibco), 1 mM sodium pyruvate (Thermo Fisher) and 10% fetal calf serum (FCS) (Gibco). Cells were cultured at 37°C, 5% CO2. To induce macrophage trans-differentiation, BLaER1 cells were cultured for six days in trans-differentiation medium, consisting of complete RPMI 1640 with 10 ng/mL IL-3 (PeproTech), 10 ng/mL M-CSF (Miltenyi) and 200 nM β-estradiol (Sigma Aldrich). Fresh trans-differentiation medium was added on day three of the trans-differentiation. On day five, cells were lifted from the culture flask using TrypLE Express (Thermo Fisher), density normalized, and replated for experiment specific purposes on day six and onwards. Stimulations were done with 10 ng/mL LPS (InvivoGen) or 1 µg/mL R848 (InvivoGen). The datasets include: Paired-end fastq.gz processed .bam Raw gene couns (Ensembl) Normalized expression values (TPM) Sample annotation metadata including cell state, stimulation condition, replicate, and associated file information