Effects of hydroxyurea on CNV induction in the mouse germline

We examined the effects of hydroxyurea (HU) treatment on germline copy number variants (CNVs) in vivo in male mice. Several trials of HU administration were performed by oral gavage and subcutaneous pump, with CNVs characterized in C57BL/6 x C3H/HeJ hybrid mouse offspring by microarray and mate-pair sequencing. This accession contains the Nimblegen aCGH microarray data from mice treated by oral gavage or by subcutaneous pump. Comparison of genomes from individual pups to all other pups from C3H/HeJ x C57BL/6 crosses, to detect de novo CNVs. C57BL/6 males were treated with hydroxyurea or served as untreated controls, while C3H/HeJ females were untreated.