Marine Metagenomics for Monitoring the Coastal Microbiota from the Ministry of Agriculture, Forestry and Fisheries of Japan

The detection sensitivity of the Kaneka DNA chromatography chip (KDCC) was evaluated with two different techniques, i.e. a multiplex-PCR and a digital DNA hybridization by digital DNA chip analyzer (DDCA), using natural plankton assemblages, i.e. amplicon sequences data of 18S-rRNA gene for metagenome data in marine eukaryotic species. There was no significant difference in the detection sensitivity among the three techniques, suggesting KDCC can be readily used to monitor the harmful algal bloom causing species.