Empirically evaluated degenerate oligonucleotide PCR primer pairs.
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The ten degenerate oligonucleotide primers were tested with the respective binding sites for sequencing primer HOG-Seq-A (see Table 2) attached to the 5′ end and used to amplify ten target genes in six apocritan Hymenoptera. d = degree of degeneration. Tm = approximate melting temperature [°C].
创建时间：
2015-12-02
