Studies on bleomycin-induced lung fibrosis in mice

We perfomed a microRNA microarray on to assess differentially expressed miRNAs in bleomycin-induced lung fibrosis in mice. To induce pulmonary fibrosis, belomycine (Sigma-Aldrich, St Louis, MO) was dissolved and administred intratracheally at a dose of 1.5U/kg body weight. Control animals received saline only. 21 days post bleomycin treatment, mice were sacrificed and lung tissue were collected for RNA extraction and microRNA microarray. C57Bl6 mice were treated with either 1.5U/kg bw bleomycin (N=3) or saline (n=3) and after 21 days, lung tissue were dissected out and RNAs were extracted and miRNA array was performed.