A role for condensin in mediating transcriptional adaptation to environmental stimuli. [RNA-Seq]

The relationship between chromatin and transcription was investigated through condensin, a key regulator of higher order chromatin structure. We use rapid conditional condensin depletion in cell cycle-staged budding yeast S. cerevisiae cells to study condensin’s role in transcriptional regulation. We observe a large number of small gene expression changes close to condensin binding sites RNA-Seq analysis of Saccharomyces cerevisiae W303-1A strains transformed with promoter exchange of the YCG1 promoter with the MET3 promoter and auxin-inducible degron (IAA7) tagged YCG1, OsTIR1 ligase or control strains . 3 biological replicates for each sample type and condition are analysed. For each biological replicate, cells were grown overnight in YNB + CSM -methioninie media, synchronised from an OD600 of 0.2 in G1 for 1.5 hours, and transitioned into YPD medium (containting methionine) to shut off the MET3 promoter and indole-acetic acid (IAA, auxin) at a final concentration of 88μg/ml to degrade Ycg1. The control and degron strains were treated indentically. Cells was collected in a G1 arrest 2 hours after condensin depletion. The cells were released into fresh YPD medium containing auxin and nocodozole, and after 2 hours the metaphase arrested cells were collected. RNA was either retained as TOTAL RNA or polyA RNA purification to generate TOTAL and POLYA RNA-Seq libraries, respectively.