UNESCO eDNA expedition in Cocos Island National Park (Costa Rica): December 2022

This dataset contains the eDNA data collected as a part of the UNESCO eDNA expeditions in Marine World Heritage Sites project in 2022-2024. The project was coordinated in collaboration with the Ocean Biodiversity Information System (OBIS) of IOC-UNESCO and the marine programme of the UNESCO World Heritage Center, and funded by the government of Flanders (Kingdom of Belgium). The samples were collected with citizen science eDNA sampling kits, containing a syringe and a filter, and preserved with Longmire's buffer, before sequencing at a central laboratory. Five different biomarkers were analysed, including 12SMifish, Mimammal, Teleo, 16S-Vert and COI markers. The sequences were analysed with the PacMAN pipeline and quality controlled including a validation check by local scientists and site managers as well as compared to existing knowledge on species distributions in OBIS and GBIF. The full sampling protocols and materials are available on the project website (https://www.unesco.org/en/edna-training-materials?hub=66910), and the bioinformatics pipeline is on github (https://github.com/iobis/PacMAN-pipeline). The data can also be explored on the eDNA expeditions dashboard (https://dashboard.ednaexpeditions.org/).本数据集收录了2022至2024年间，联合国教科文组织（UNESCO）“海洋世界遗产地环境DNA（eDNA）科考项目”所采集的环境DNA数据。该项目由联合国教科文组织政府间海洋学委员会下属的海洋生物信息系统（Ocean Biodiversity Information System, OBIS）与联合国教科文组织世界遗产中心海洋项目联合统筹，并获得比利时弗拉芒地区政府资助。本次采样采用公民科学型环境DNA采样套件完成，套件包含注射器与滤膜，样本使用朗缪尔缓冲液（Longmire's buffer）保存后，送至中央实验室开展测序。本次分析共涉及5类不同生物标记物，包括12SMifish、Mimammal、Teleo、16S-Vert及COI标记。测序序列通过PacMAN pipeline进行分析并完成质量控制，质控环节包括当地科研人员与场地管理者开展的验证核查，同时将分析结果与OBIS及全球生物多样性信息设施（Global Biodiversity Information Facility, GBIF）中已有的物种分布相关知识进行比对。完整的采样方案与配套材料可通过项目官网（https://www.unesco.org/en/edna-training-materials?hub=66910）获取，生物信息学分析流程托管于GitHub平台（https://github.com/iobis/PacMAN-pipeline）。用户还可通过环境DNA科考项目可视化仪表板（https://dashboard.ednaexpeditions.org/）浏览本数据集。