Atrazine, desethylatrazine (DEA) and desisopropylatrazine (DIA) degradation by Pleurotus ostreatus INCQS 40310

Atrazine is the most common herbicide applied in crops of economic relevance, such as sugar cane, soybean, and corn. Atrazine and its derivatives desethylatrazine (DEA) and desisopropylatrazine (DIA) are toxic to the environment, affecting animal and human health. Thus, this study aimed to evaluate the degradation of atrazine and its derivatives by the fungus Pleurotus ostreatus INCQS 40310, as well as the potential of the enzymes involved in this process. P. ostreatus INCQS 40310 was able to degrade atrazine (82%), DEA (71%), and DIA (56%) over 22 days of fungal cultivation. Proteomic analysis indicated the participation of hydrolases and peroxidases during the degradation process. Additionally, resting cells of the fungus were tested to verify the action of intracellular enzymes in the degradation process, suggesting the participation of cytochrome P450 enzymatic complex. Resting cells experiments promoted the degradation of 50% of atrazine, 36% of DIA, 30% of DEA. So far, this is the first work evaluating the biodegradation of DEA and DIA by fungus.

莠去津（Atrazine）是经济作物中应用最为广泛的除草剂，涵盖甘蔗、大豆与玉米等品类。莠去津及其衍生物脱乙基莠去津（DEA）、脱异丙基莠去津（DIA）均对环境具有毒性，会危害动物与人类健康。据此，本研究旨在评估糙皮侧耳（Pleurotus ostreatus）INCQS 40310对莠去津及其衍生物的降解效果，并探究该过程中相关酶的作用潜力。在22天的真菌培养周期内，糙皮侧耳INCQS 40310可分别降解82%的莠去津、71%的DEA以及56%的DIA。蛋白质组学分析显示，降解过程中有水解酶与过氧化物酶参与其中。此外，本研究通过真菌静息细胞实验验证了胞内酶在降解过程中的作用，结果表明细胞色素P450酶复合物参与了该降解过程。静息细胞实验可实现50%的莠去津、36%的DIA以及30%的DEA降解。截至目前，本研究是首个针对真菌降解DEA与DIA的生物降解作用开展的相关工作。