MES bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation

MES bound form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H and S176A inactivation Descriptor: 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, Alpha/beta hydrolase family protein, CALCIUM ION, ... Authors: Emori, M, Numoto, N, Kamiya, N, Oda, M. Deposit date: 2023-02-10 Release date: 2023-03-15 Last modified: 2024-10-23 Method: X-RAY DIFFRACTION (2.6 Å) Cite: Improvement of thermostability and activity of PET-degrading enzyme Cut190 towards a detailed understanding and application of the enzymatic reaction mechanism. Biorxiv, 2023

带有热稳定性提升突变（S226P/R228S/Q138A/D250C-E296C/Q123H/N202H）且引入S176A失活突变的聚对苯二甲酸乙二醇酯（Polyethylene Terephthalate，简称PET）降解角质酶Cut190的MES结合态形式。描述符：2-(N-吗啉代)乙磺酸（2-(N-MORPHOLINO)-ETHANESULFONIC ACID，简称MES）、α/β水解酶家族蛋白、钙离子（CALCIUM ION）等。作者：Emori M、Numoto N、Kamiya N、Oda M。入库日期：2023年2月10日；发布日期：2023年3月15日；最后更新日期：2024年10月23日；实验方法：X射线衍射（分辨率2.6埃）；引用文献：《PET降解酶Cut190的热稳定性与活性改良：深入解析酶促反应机制及其应用》，BioRxiv，2023年。