High-resolution metaproteomic analysis of an ocean microbiome preceding a Chaetoceros bloom

From May 27 - June 18, 2021, marine microbial samples were collected in East Sound, WA, USA every 4 hours. This high temporal resolution sampling effort captured the initiation of two separate Chaetoceros socialis blooms. A metaproteomic data independent analysis was performed on the 6 days of the time series preceding the second bloom (June 12 – 18). A subsequent targeted analysis using parallel reaction monitoring was performed on the time period preceding the first bloom (May 28 – June 2). Prior to the PRM analysis, a matrix-matched calibration curve was analyzed to assess if peptides from a representative microbiome sample could be accurately quantified.