Early Transcriptional Responses to Human Paratyphoid Challenge

The whole blood transcriptome 12 hours after challenge with S. Paratyphi A. Samples were collected in a study aiming to find the challenge dose giving a 60-75% attack rate, published in Dobinson et al. PMID: 28158395. Participants were challenged with S. Paratyphi A NVGH308 strain suspended in sodium bicarbonate. Enteric fever was diagnosed based on oral temperature or positive blood culture. Participants were treated with antibiotics either following diagnosis or 14 days without diagnosis. Paired baseline samples are available under Series GSE114033. Overall design: Outpatient dose-finding challenge study in healthy human volunteers. Peripheral blood was collected 12 hours post-challenge in Tempus Blood RNA tubes. RNA was extracted using a Tempus Spin RNA Isolation kit and poly-A selected.