Deciphering protective mechanism against human type 2 diabetes through in vitro ß cell differentiation

To systemically investigate the role of ZnT8 in ß cell maturation, we performed single cell RNA-seq in both WT and KO ß cells at both S6 (immature) and S7 (mature) stages. Both WT and KO ß cells were obtained from FACS as positive for both INS and NKX6.1. Single cell RNA-seq results revealed that SLC30A8 is mainly involved in ß cell maturation process, and further showed that SLC30A8 LOF accelerates ß cell maturation and upregulates insulin secretion pathway in mature ß cells. Overall design: In total, 1543 cells with four types of ß cell were utilized.