Annotation spreadsheet for transcriptome assembly of Lithobates clamitans
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An Excel spreadsheet of contig sequence similarity for the Lithobates clamitans assembly. BLASTX hits to Xenopus tropicalis, HMMER matches to the Pfam-A database (for predicted ORFs), and other annotation information is given. The column headings are as follows: Contig: contig name Length (bp): length in base pairs %GC content: percent of contig sequence that is C or G Longest ORF (min 50 amino acids): the longest ORF identified on the contig that was at least 50 amino-acids long Pfam (E-value threshold 0.1): A colon-delimited list of matched Pfam accessions, E-values, and domain descriptions Xenopus.tropicalis BLASTX E-value: E-value of the best BlastX match to X. tropicalis proteins. bit: bit score of this match id%: percentage identity (protein sequence) of this match to the query contig description: descriptive text in the fasta header of the best match RefSeq ID: associated RefSeq ID for best match Entrez gene ID: associated Entrez gene ID for best match UniProt ID: associated UniProt ID for best match
本数据集为绿臭蛙（*Lithobates clamitans*）基因组组装的重叠群序列相似性分析Excel表格，其中包含与热带爪蟾（*Xenopus tropicalis*）的BLASTX比对结果、针对预测开放阅读框（Open Reading Frame, ORF）的Pfam-A数据库HMMER匹配结果，以及其他相关注释信息。各列字段说明如下： Contig：重叠群名称 Length (bp)：序列长度（单位：碱基对，bp） %GC content：重叠群序列的GC含量百分比 Longest ORF (min 50 amino acids)：该重叠群上鉴定到的最长开放阅读框，长度不低于50个氨基酸 Pfam (E-value threshold 0.1)：以冒号分隔的匹配Pfam条目编号、E值及结构域描述列表 Xenopus.tropicalis BLASTX E-value：与热带爪蟾蛋白质序列进行BLASTX比对所得最优匹配的E值 bit：该最优比对的比特得分 id%：该最优比对与查询重叠群的蛋白质序列一致性百分比 description：最优比对结果的FASTA头部描述文本 RefSeq ID：最优比对结果对应的RefSeq编号 Entrez gene ID：最优比对结果对应的Entrez基因编号 UniProt ID：最优比对结果对应的UniProt编号
