File S1 - Secretoneurin Gene Therapy Improves Blood Flow in an Ischemia Model in Type 1 Diabetic Mice by Enhancing Therapeutic Neovascularization

Supporting text and figures. Figure S1. SN effect on mRNA expression of growth factors/receptors under high glucose. None of the analyzed growth factors or their corresponding receptors were significantly regulated on mRNA level by stimulation with SN under high glucose conditions. The following growth factors and growth factor receptors have been investigated: VEGF (A), VEGF receptor 2 (VEGFR2) (B), basic fibroblast growth factor (bFGF) (C), FGF receptor 3 (FGFR3) (D), insulin-like growth factor receptor (IGFR) (E) and epidermal growth factor receptor (EGFR) (F). Figure S2. SN effect on mRNA expression of TXNIP and PIM1 under high glucose. (A) shows thioredoxin-interacting protein (TXNIP) mRNA levels of HUVECS under high glucose and stimulation with SN 10 ng/ml for 12 hours. SN showed no effect on TXNIP or Proto-oncogene serine/threonine-protein kinase (PIM1) mRNA expression (B). SN showed no effect on TIXNIP expression under stimulation by SN in combination with VEGF (C).
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