Gene expression profile at single cell level of small intestine epithelial cells from the distal ileum. Gene expression profile at single cell level of small intestine epithelial cells from the distal ileum

Small intestine is highly innervated by nociceptor neurons. Single cell RNA sequencing (scRNA-seq) of ileal epithelial cells was performed to analyze the role of nociceptor neurons in regulating small intestinal epithelial cells. Overall design: Ileal epithelial cells were purified by Fluorescence-activated cell sorting (FACS) from both nociceptor knockout (Nav1.8Cre_P_DTA_P) mice and the control (Nav1.8Cre_M_DTA_P) mice. The purified cells were used for scRNAseq using inDrops.