Human primary fetal lung fibroblasts (HFL-1) undergoing replicative senescence

We profiled the global gene expression of human primary fetal lung fibroblasts (HFL-1) at different stages while they were undergoing replicative senescence We maintained human primary fetal lung fibroblasts (HFL-1) in culture from a young stage until they reached a senescent stage after 50 replication cycles. The cells were collected at three different time points while undergoing RS. In the first young stage the cells were replicating every 24 hours, in the second middle-aged stage the cells were replicating every 7 days and in the third stage the cells have not replicated for 14 days.