Supplementary Figure S1 GSEA and ssGSEA analysis

Bubble plots exhibited the top-ranked GSEA results for the top-ranked KEGG pathways in (A) recurrent POP compared with primary POP uterosacral ligaments, and (B) recurrent POP compared with non-POP uterosacral ligaments. The classic GSEA plots showed that both adipose- and inflammation-related pathways were activated in the two contrast matrices for (C) recurrent POP vs.vs primary POP uterosacral ligaments, and (D) recurrent POP vs.vs non-POP uterosacral ligaments. Heatmaps showed hierarchical clustering of ssGSEA scores of KEGG pathways differentially enriched in (E) recurrent POP vs.vs primary POP uterosacral ligaments and (F) recurrent POP vs.vs non-POP uterosacral ligaments. (G) Spearman correlation analysis of pathway ssGSEA scores revealed that PPAR signaling pathways was strongly associated with adipose- and inflammation-related pathways in non-POP, primary POP and recurrent POP uterosacral ligaments. GSEA, g: Gene set enrichment analysis; ssGSEA, the s: Single-sample gene set enrichment analysis; POP, p: Pelvic organ prolapses; NES, n: Normalized enrichment scores.