RNA-Seq analysis of prostate cancer cell line PC-3 treated with siRNA control (siCont) and siELL2

To identify the genes associated with the knockdown of ELL2. PC-3 mRNA profiles of cells treated with RNA interference ELL2 compared to non-targeted control siRNA were tested using RNA seq.  RNA isolation was performed using RNeasy Plus Mini kit. In this study, we identified 1939 differentially expressed genes associated with ELL2 deletion. Of these transcripts,1579 genes involve in cancer, while 695 genes are asscoicated with cell death and survival. Bioinformatics analysis indicated that ELL2 knockdown activates the IFNγ-STAT1-IRF1 signaling pathway, which is known to play important roles in prostate cancer. PC-3  cells were seeded in a 6-well plate at density of 3x10^5 per well. Cells were treated with 25nM siELL2 or siControl for 72 hour in standard culture condition and then were harvested for RNA extraction.