M1 and M2 myeloid cells behave differently in classical immune type contexts

Macrophages maintain tissue homeostasis and act as first responders to immune threats. A central question is whether these cells exhibit transcriptional plasticity in vivo. We tested macrophage polarization across classical type 4 immunity using a B16F10 melanoma model. Overall design: Single-cell RNA sequencing data were obtained from mouse pulmonary cells 16 days post-iv injection of 400,000 B16F10 melanoma cells. Three samples were loaded on separate lanes, where each loaded sample contained 3 pooled lungs.