Global Gene Expression of H9 hESC with different passage numbers. Global Gene Expression of H9 hESC with different passage numbers

Transcriptome profile was obtained from a set of human embryonic stem cell (hESCs) line (WA09: H9) with different passage numbers (P1: 40s, P2: 100s, P3: 200s, P4: 300s passage). Culture adaptation occurs in hESCs during repeated in vitro culture to acquire ‘survival advantage’ to be highly resistant to various stresses. In special, difference in gene expression profile of cell death or apoptotic gene signature was evident between P1/P2 and P3/P4 hESCs. Overall design: Microarray analysis of human embryonic stem cells (hESCs) with different passage numbers P1: H9 hESCs (40s passage), P2: H9 hESCs (100s passage), P3: H9 hESCs (200s passage), P4: H9 hESCs (300s passage)