Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil

ABSTRACT: The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra.

摘要：本研究旨在评估产自巴西半干旱地区的詹代拉无刺蜂（stingless bee, SB，Melipona subnitida）所产蜂蜜中的微生物存在情况。本研究共分析35份蜂蜜样本，所有样本均检出嗜温菌、45℃大肠菌群、真菌及酵母菌。85.7%的样本中检出葡萄球菌属（Staphylococcus spp.），34.3%的样本中检出芽孢杆菌属（Bacillus spp.）。分别有40%和2.8%的样本检出产气荚膜梭菌（Clostridium perfringens）与肉毒梭菌（C. botulinum）的DNA。未检出沙门氏菌属（Salmonella spp.）与艰难梭菌（C. difficile）。本研究揭示了詹代拉无刺蜂所产蜂蜜中存在丰富的微生物多样性。