COMP-Ang1 protein localization in the tPA-4h group visualized with a confocal laser microscope.

From left to right: markers of cells that make up the blood brain barrier (a, e, i; red), neuronal cell (m; red), COMP-Ang1 protein (b, f, j, n; green), DAPI stain (c, g, k, o; blue), and a merged image (d, h, l, p). Immunostaining with an anti-FLAG antibody was performed on COMP-Ang1 proteins in order to differentiate them from endogenous Ang1. RECA1 is an endothelial cell marker protein; PDGFRβ is a pericyte marker; GFAP is an astrocyte marker; and MAP2 is a neuronal cell marker. RECA1, rat endothelial cell antigen; PDGFRβ, platelet-derived growth factor receptor; GFAP, glial fibrillary acidic protein; Ang1, angiopoietin-1. The scale bars are 10 µm.