Replication Data for: Advancing Quantification methods for Polyphenols in Brown Seaweeds - applying a Selective qNMR Method compared with the TPC Assay

These data belong to the comprehensive study of polyphenols in Laminaria hyperborea, Laminaria digitata, Saccharina latissima, Ascophyllum nodosum and Fucus vesiculosus performed in relation with the publication titled: \"Advancing Quantification methods for Polyphenols in Brown Seaweeds - applying a Selective qNMR Method compared with the TPC Assay\". The dataset contains 22 files, describing the quantitative content of polyphenols in the brown seaweeds Laminaria hyperborea, Laminaria digitata, Saccharina latissima, Ascophyllum nodosum and Fucus vesiculosus. The quantitative content was determined by Optimized Total Phenolic Content (TPC) assay and quantitative NMR (qNMR). Publication abstract: Polyphenols are important components of seaweeds contributing to their potential use in food-, feed- and health applications. In this study, optimized methods of the Folin-Ciocalteu total phenolic content assay (TPC) and selective quantitative 1H NMR (qNMR), including 13C NMR characterization, were compared for selected brown seaweeds from sublittoral to intertidal zone (Laminaria hyperborea, Laminaria digitata, and Fucus vesiculosus). TPC and selective qNMR resulted in comparable quantities for the sublittoral growing Laminariaceae, with lower polyphenolic content (TPC: 0.6–0.7%; qNMR: 0.7–0.8%, GAE, DW). F. vesiculosus, found in more shallow waters with a higher polyphenolic production, yields a TPC quantification more than double the qNMR quantification (TPC: 3.7%; qNMR: 1.5% GAE, DW). This difference most likely reflects the lack of sensitivity in the TPC assay concerning species with interfering compounds and/or a higher polyphenolic content, and conceivably also a higher diversity of polyphenols (> 1 % of biomass). From the 13C NMR characterization, L. hyperborea, L. digitata, and F. vesiculosus all showed a larger proportion of phlorethol-like phlorotannins compared to fucol-type phlorotannins.本数据集隶属于针对北极海带（Laminaria hyperborea）、掌状海带（Laminaria digitata）、长叶海带（Saccharina latissima）、囊藻（Ascophyllum nodosum）以及泡叶藻（Fucus vesiculosus）中多酚类物质的综合性研究，相关研究成果已发表于题为《推进褐藻多酚定量方法：选择性qNMR法与TPC测定法的对比应用》的学术论文中。 本数据集包含22个文件，详细记载了上述五种褐藻的多酚类物质定量含量，该定量结果通过优化后的总酚含量（Total Phenolic Content, TPC）测定法与定量核磁共振（quantitative NMR, qNMR）法测得。 论文摘要：多酚是褐藻的关键组成成分，在食品、饲料及健康相关领域具备潜在应用价值。本研究针对潮下带至潮间带区域的五种褐藻（北极海带Laminaria hyperborea、掌状海带Laminaria digitata、长叶海带Saccharina latissima、囊藻Ascophyllum nodosum、泡叶藻Fucus vesiculosus），对比分析了优化后的福林-酚总酚含量（TPC）测定法与选择性定量1H核磁共振（1H qNMR）法（含13C核磁共振表征）的定量效果。 结果显示，潮下带生长的海带科藻类的TPC与qNMR定量结果相近，多酚含量较低（TPC：0.6–0.7%；qNMR：0.7–0.8%，以没食子酸当量（Gallactic Acid Equivalent, GAE）、干重计）。栖息于更浅水域、多酚合成水平更高的泡叶藻Fucus vesiculosus，其TPC定量结果约为qNMR定量结果的两倍以上（TPC：3.7%；qNMR：1.5% GAE，干重计）。 该差异大概率反映了TPC测定法对存在干扰化合物的物种和/或多酚含量较高的物种灵敏度不足，同时也可能与多酚类物质多样性更高（占生物量1%以上）有关。通过13C核磁共振表征分析发现，北极海带Laminaria hyperborea、掌状海带Laminaria digitata与泡叶藻Fucus vesiculosus中，类间苯三酚型褐藻单宁（phlorethol-like phlorotannins）的占比均高于岩藻醇型褐藻单宁（fucol-type phlorotannins）。