Protein composition of extracellular vesicles isolated from Haloferax volcanii

In order to understand extraceluular vesicle (EV) production in Euryarchaea, and in particular halophilic archaea, we used the model archaeal organism, Haloferax volcanii, to investigate the composition of EVs as well as their capacity to transfer their cargo to other organisms. EVs were isolated in tripclicates and purified with a density gradient. Both of two bands (upper and lower band) were isolated from the gradient and analysed seperately. Additionally, cellular membranes were isolated in triplicates and analysed for their protein content, to allow conclusions about proteins that are preferentially enclosed in EVs.