Multi-omics integration identifies progenitor-state specific PBRM1-PIAS1 cooperation in repressing differentiation in epithelial tissue

Here we determine the gene targets controlled by PBRM1-PIAS1 cooperation in regulating epidermal differentiation. RNA-seq for PBRM1 shRNA knockdown and PIAS1 shRNA knockdown was performed on undifferentiated and differentiated keratinocytes against controls. ChIP-seq for PBRM1 and PIAS1 was performed in undifferentiated and differentiated keratinocytes. CUT&RUN for BRD7 was performed in undifferentiated keratinocytes in control versus PBRM1 shRNA knockdown conditions.