Unraveling CD4+ T Cell Diversity in Abdominal Aortic Aneurysms

We conducted single-cell RNA sequencing (scRNA-seq) on CD4+ T cells of the aneurysmal aorta and the corresponding splenic cells, in order to unveil the diversity of CD4+ T Cell in Abdominal Aortic Aneurysms. The mouse model of abdominal aortic aneurysm was established, and the spleen was sampled 14 days later to prepare a single cell suspension. CD4+ T cells were sorted by flow cytometry and subsequently analyzed using scRNA-seq.